› 1v7r

X-ray diffraction
1.4Å resolution

Structure of nucleotide triphosphate pyrophosphatase from pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb1v7r/pdb
PDB entry 1v7r coloured by chain, front view.
PDB entry 1v7r coloured by chain, side view.
PDB entry 1v7r coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Structures of dimeric nonstandard nucleotide triphosphate pyrophosphatase from Pyrococcus horikoshii OT3: functional significance of interprotomer conformational changes.
Lokanath NK, Pampa KJ, Takio K, Kunishima N
J Mol Biol 375 1013-25 (2008)
PMID: 18062990

Function and Biology Details

Reaction catalysed: 
ITP + H(2)O = IMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129876 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dITP/XTP pyrophosphatase Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 21.23 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O59580 (Residues: 1-186; Coverage: 100%)
Gene name: PH1917
Sequence domains: Ham1 family
Structure domains: Maf protein

Ligands and Environments

1 bound ligand:
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: C2221
Unit cell:
a: 79.013Å b: 93.188Å c: 55.621Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Preventive DNA repair by sanitizing the cellular (deoxy)nucleoside triphosphate pool.
Nagy et al. (2014)
2 other articles

3 mentions without citation

ITPA (inosine triphosphate pyrophosphatase): from surveillance of nucleotide pools to human disease and pharmacogenetics.
Simone et al. (2013)
2 more