› 1v93

X-ray diffraction
1.9Å resolution

5,10-Methylenetetrahydrofolate Reductase from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb1v93/pdb
PDB entry 1v93 coloured by chain, front view.
PDB entry 1v93 coloured by chain, side view.
PDB entry 1v93 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Nakajima Y, Miyahara I, Hirotsu K, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
5-methyltetrahydrofolate + NAD(+) = 5,10-methylenetetrahydrofolate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylenetetrahydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 33.05 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RA47 (Residues: 1-216; Coverage: 94%)
Gene name: metF
Sequence domains: Methylenetetrahydrofolate reductase
Structure domains: TIM Barrel

Ligands and Environments

2 bound ligands:
Ligand FAD 1 x FAD
Ligand DIO 6 x DIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: I23
Unit cell:
a: 123.937Å b: 123.937Å c: 123.937Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.236
Expression system: Escherichia coli

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