› 1v96

X-ray diffraction
1.75Å resolution

Crystal structure of hypothetical protein of unknown function from pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb1v96/pdb
PDB entry 1v96 coloured by chain, front view.
PDB entry 1v96 coloured by chain, side view.
PDB entry 1v96 coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
Structure of PIN-domain protein PH0500 from Pyrococcus horikoshii.
Jeyakanthan J, Inagaki E, Kuroishi C, Tahirov TH
Acta Crystallogr Sect F Struct Biol Cryst Commun 61 463-8 (2005)
PMID: 16511069

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease VapC4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 149 amino acids
Theoretical weight: 17.21 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O58236 (Residues: 1-149; Coverage: 100%)
Gene names: PH0500, vapC4
Structure domains: 5'-nuclease

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 44.909Å b: 95.463Å c: 102.656Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.228
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structural conservation of the PIN domain active site across all domains of life.
Senissar et al. (2017)
11 other articles

3 mentions without citation

The crystal structure of the Rv0301-Rv0300 VapBC-3 toxin-antitoxin complex from M. tuberculosis reveals a Mg²⁺ ion in the active site and a putative RNA-binding site.
Min et al. (2012)
2 more