› 1v9z

X-ray diffraction
1.9Å resolution

Crystal Structure of the heme PAS sensor domain of Ec DOS (Ferrous Form)

Released:
DOI: 10.2210/pdb1v9z/pdb
PDB entry 1v9z coloured by chain, front view.
PDB entry 1v9z coloured by chain, side view.
PDB entry 1v9z coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
A redox-controlled molecular switch revealed by the crystal structure of a bacterial heme PAS sensor.
Kurokawa H, Lee DS, Watanabe M, Sagami I, Mikami B, Raman CS, Shimizu T
J Biol Chem 279 20186-93 (2004)
PMID: 14982921

Function and Biology Details

Reaction catalysed: 
Cyclic di-3',5'-guanylate + H(2)O = 5'-phosphoguanylyl(3'->5')guanosine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxygen sensor protein DosP Chains: A, B
Molecule details ›
Chains: A, B
Length: 167 amino acids
Theoretical weight: 19.02 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P76129 (Residues: 1-139; Coverage: 17%)
Gene names: JW1484, b1489, dos, dosP, pdeO, yddU
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 46.4Å b: 69.18Å c: 82.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.244 0.287
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Ligand-binding PAS domains in a genomic, cellular, and structural context.
Henry et al. (2011)
11 more

7 mentions without citation

Structure and signaling mechanism of Per-ARNT-Sim domains.
Möglich et al. (2009)
6 more