› 1va6

X-ray diffraction
2.1Å resolution

Crystal structure of Gamma-glutamylcysteine synthetase from Escherichia Coli B complexed with Transition-state analogue

Released:
DOI: 10.2210/pdb1va6/pdb
PDB entry 1va6 coloured by chain, front view.
PDB entry 1va6 coloured by chain, side view.
PDB entry 1va6 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of gamma-glutamylcysteine synthetase: insights into the mechanism of catalysis by a key enzyme for glutathione homeostasis.
Hibi T, Nii H, Nakatsu T, Kimura A, Kato H, Hiratake J, Oda J
Proc Natl Acad Sci U S A 101 15052-7 (2004)
PMID: 15477603

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141436 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate--cysteine ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 518 amino acids
Theoretical weight: 58.27 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6W9 (Residues: 1-518; Coverage: 100%)
Gene names: JW2663, b2688, gsh-I, gshA
Sequence domains: Glutamate-cysteine ligase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 8 x MG
Ligand P2S 2 x P2S
Ligand ADP 2 x ADP
Ligand P6G 2 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 70.468Å b: 97.36Å c: 102.185Å
α: 90° β: 109.63° γ: 90°
R-values:
R R work R free
0.2 0.2 0.225
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Redox-directed cancer therapeutics: molecular mechanisms and opportunities.
Wondrak GT. (2009)
11 more

8 mentions without citation

Molecular analysis of the prokaryotic ubiquitin-like protein (Pup) conjugation pathway in Mycobacterium tuberculosis.
Cerda-Maira et al. (2010)
7 more