› 1vah

X-ray diffraction
2.4Å resolution

Crystal structure of the pig pancreatic-amylase complexed with r-nitrophenyl-a-D-maltoside

Released:
DOI: 10.2210/pdb1vah/pdb
PDB entry 1vah coloured by chain, front view.
PDB entry 1vah coloured by chain, side view.
PDB entry 1vah coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Crystal structure of the pig pancreatic alpha-amylase complexed with rho-nitrophenyl-alpha-D-maltoside-flexibility in the active site.
Zhuo H, Payan F, Qian M
Protein J 23 379-87 (2004)
PMID: 15517985

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pancreatic alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 496 amino acids
Theoretical weight: 55.47 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00690 (Residues: 16-511; Coverage: 100%)
Gene name: AMY2
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand CA 1 x CA
Ligand NPO 1 x NPO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 56.3Å b: 87.8Å c: 103.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.17 0.233

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Citations

1 review citation

Polyphenolic Compounds and Digestive Enzymes: In Vitro Non-Covalent Interactions.
Martinez-Gonzalez et al. (2017)
5 other articles

5 mentions without citation

Functional constituents of wild and cultivated Goji (L. barbarum L.) leaves: phytochemical characterization, biological profile, and computational studies.
Mocan et al. (2017)
4 more