› 1vav

X-ray diffraction
2Å resolution

Crystal structure of alginate lyase PA1167 from Pseudomonas aeruginosa at 2.0 A resolution

Released:
DOI: 10.2210/pdb1vav/pdb
PDB entry 1vav coloured by chain, front view.
PDB entry 1vav coloured by chain, side view.
PDB entry 1vav coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structure and function of a hypothetical Pseudomonas aeruginosa protein PA1167 classified into family PL-7: a novel alginate lyase with a beta-sandwich fold.
Yamasaki M, Moriwaki S, Miyake O, Hashimoto W, Murata K, Mikami B
J Biol Chem 279 31863-72 (2004)
PMID: 15136569

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191367 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alginate lyase 2 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 25.02 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I4H0 (Residues: 2-223; Coverage: 100%)
Gene name: PA1167
Sequence domains: Alginate lyase
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 43.359Å b: 70.219Å c: 67.299Å
α: 90° β: 94.77° γ: 90°
R-values:
R R work R free
0.187 0.187 0.24
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structural and mechanistic classification of uronic acid-containing polysaccharide lyases.
Garron et al. (2010)
5 more

9 mentions without citation

Diversity of Three-Dimensional Structures and Catalytic Mechanisms of Alginate Lyases.
Xu et al. (2018)
8 more