› 1vb5

X-ray diffraction
2.2Å resolution

Crystal Structure Analysis of the Pyrococcus horikoshii OT3 translation initiation factor eIF-2B

Released:
DOI: 10.2210/pdb1vb5/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1vb5 coloured by chain, front view.
PDB entry 1vb5 coloured by chain, side view.
PDB entry 1vb5 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Crystal structure of the regulatory subunit of archaeal initiation factor 2B (aIF2B) from hyperthermophilic archaeon Pyrococcus horikoshii OT3: a proposed structure of the regulatory subcomplex of eukaryotic IF2B.
Kakuta Y, Tahara M, Maetani S, Yao M, Tanaka I, Kimura M
Biochem Biophys Res Commun 319 725-32 (2004)
PMID: 15184043

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-129676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative translation initiation factor eIF-2B subunit 2-like Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 31.44 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58185 (Residues: 1-276; Coverage: 100%)
Gene name: PH0440
Sequence domains: Initiation factor 2 subunit family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P6322
Unit cell:
a: 158.437Å b: 158.437Å c: 151.486Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.209 0.234
Expression system: Escherichia coli

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Citations

3 review citations

Translational regulation of GCN4 and the general amino acid control of yeast.
Hinnebusch AG. (2005)
2 more

5 mentions without citation

Architecture of the eIF2B regulatory subcomplex and its implications for the regulation of guanine nucleotide exchange on eIF2.
Kuhle et al. (2015)
4 more