› 1vbr

X-ray diffraction
1.8Å resolution

Crystal structure of complex xylanase 10B from Thermotoga maritima with xylobiose

Released:
DOI: 10.2210/pdb1vbr/pdb
PDB entry 1vbr coloured by chain, front view.
PDB entry 1vbr coloured by chain, side view.
PDB entry 1vbr coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structural basis of the substrate subsite and the highly thermal stability of xylanase 10B from Thermotoga maritima MSB8.
Kumasaka T, Kaneko T, Morokuma C, Yatsunami R, Sato T, Nakamura S, Tanaka N
Proteins 61 999-1009 (2005)
PMID: 16247799

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-xylanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 38.7 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WXS5 (Residues: 20-347; Coverage: 95%)
Gene name: TM_0070
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP, XYS
Carbohydrate polymer
1 bound ligand:
Ligand ACY 6 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P1
Unit cell:
a: 58.46Å b: 58.43Å c: 61.29Å
α: 70.43° β: 84.33° γ: 68.77°
R-values:
R R work R free
0.194 0.181 0.215
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Thermostable enzymes as biocatalysts in the biofuel industry.
Yeoman et al. (2010)
1 more

8 mentions without citation

Terminal amino acids disturb xylanase thermostability and activity.
Liu et al. (2011)
7 more