› 1vdc

X-ray diffraction
2.5Å resolution

STRUCTURE OF NADPH DEPENDENT THIOREDOXIN REDUCTASE

Released:
DOI: 10.2210/pdb1vdc/pdb
PDB entry 1vdc coloured by chain, front view.
PDB entry 1vdc coloured by chain, side view.
PDB entry 1vdc coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structure of Arabidopsis thaliana NADPH dependent thioredoxin reductase at 2.5 A resolution.
Dai S, Saarinen M, Ramaswamy S, Meyer Y, Jacquot JP, Eklund H
J Mol Biol 264 1044-57 (1996)
PMID: 9000629

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174440 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase 1, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 333 amino acids
Theoretical weight: 35.42 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q39243 (Residues: 43-375; Coverage: 89%)
Gene names: At4g35460, F15J1.30, NTR1, NTRB
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG
Spacegroup: P41212
Unit cell:
a: 89.974Å b: 89.974Å c: 175.626Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.243
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Redox regulation: a broadening horizon.
Buchanan et al. (2005)
11 more

8 mentions without citation

Structure of the yeast vacuolar ATPase.
Zhang et al. (2008)
7 more