› 1vdk

X-ray diffraction
1.8Å resolution

Crystal structure of fumarase from thermus thermophilus HB8

Released:
DOI: 10.2210/pdb1vdk/pdb
PDB entry 1vdk coloured by chain, front view.
PDB entry 1vdk coloured by chain, side view.
PDB entry 1vdk coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Mizutani H, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase class II Chains: A, B
Molecule details ›
Chains: A, B
Length: 466 amino acids
Theoretical weight: 50.94 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84127 (Residues: 1-466; Coverage: 100%)
Gene names: fumC, tthHB8IM
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2221
Unit cell:
a: 125.336Å b: 140.122Å c: 135.372Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.201
Expression system: Escherichia coli BL21(DE3)

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