› 1ve9

X-ray diffraction
2.5Å resolution

Porcine kidney D-amino acid oxidase

Released:
DOI: 10.2210/pdb1ve9/pdb
PDB entry 1ve9 coloured by chain, front view.
PDB entry 1ve9 coloured by chain, side view.
PDB entry 1ve9 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Three-dimensional structure of porcine kidney D-amino acid oxidase at 3.0 A resolution.
Mizutani H, Miyahara I, Hirotsu K, Nishina Y, Shiga K, Setoyama C, Miura R
J Biochem 120 14-7 (1996)
PMID: 8864836

Function and Biology Details

Reaction catalysed: 
A D-amino acid + H(2)O + O(2) = a 2-oxo carboxylate + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-amino-acid oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 39.38 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00371 (Residues: 1-347; Coverage: 100%)
Gene name: DAO
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
Ligand BEZ 2 x BEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 110.3Å b: 92.9Å c: 71.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.254
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Sequence-structure analysis of FAD-containing proteins.
Dym et al. (2001)
11 more

8 mentions without citation

Crystal structure of human D-amino acid oxidase: context-dependent variability of the backbone conformation of the VAAGL hydrophobic stretch located at the si-face of the flavin ring.
Kawazoe et al. (2006)
7 more