› 1vfb

X-ray diffraction
1.8Å resolution

BOUND WATER MOLECULES AND CONFORMATIONAL STABILIZATION HELP MEDIATE AN ANTIGEN-ANTIBODY ASSOCIATION

Released:
DOI: 10.2210/pdb1vfb/pdb
PDB entry 1vfb coloured by chain, front view.
PDB entry 1vfb coloured by chain, side view.
PDB entry 1vfb coloured by chain, top view.
Source organisms:
Primary publication:
Bound water molecules and conformational stabilization help mediate an antigen-antibody association.
Bhat TN, Bentley GA, Boulot G, Greene MI, Tello D, Dall'Acqua W, Souchon H, Schwarz FP, Mariuzza RA, Poljak RJ
Proc Natl Acad Sci U S A 91 1089-93 (1994)
PMID: 8302837

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-132833 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin kappa chain variable 12-41 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01635 (Residues: 21-115; Coverage: 100%)
Gene names: Gm16848, Igkv12-41
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig heavy chain V region PJ14 Chain: B
Molecule details ›
Chain: B
Length: 116 amino acids
Theoretical weight: 12.86 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01820 (Residues: 20-115; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Lysozyme C Chain: C
Molecule details ›
Chain: C
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Not provided
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 129.2Å b: 60.8Å c: 56.9Å
α: 90° β: 119.3° γ: 90°
R-values:
R R work R free
0.185 0.185 not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

36 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
35 more

59 mentions without citation

Molecular basis for the preferential cleft recognition by dromedary heavy-chain antibodies.
De Genst et al. (2006)
58 more