1vfg

X-ray diffraction
2.8Å resolution

Crystal structure of tRNA nucleotidyltransferase complexed with a primer tRNA and an incoming ATP analog

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-130372 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
A-adding tRNA nucleotidyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 390 amino acids
Theoretical weight: 45.72 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66728 (Residues: 443-824; Coverage: 46%)
Gene name: aq_411
Sequence domains: Poly A polymerase head domain
Structure domains:
RNA (75-MER) Chains: C, D
Molecule details ›
Chains: C, D
Length: 75 nucleotides
Theoretical weight: 24.16 KDa
Source organism: Thermotoga maritima
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P2
Unit cell:
a: 109.476Å b: 125.9Å c: 58.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.286
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided