› 1vfy

X-ray diffraction
1.15Å resolution

PHOSPHATIDYLINOSITOL-3-PHOSPHATE BINDING FYVE DOMAIN OF VPS27P PROTEIN FROM SACCHAROMYCES CEREVISIAE

Released:
DOI: 10.2210/pdb1vfy/pdb
PDB entry 1vfy coloured by chain, front view.
PDB entry 1vfy coloured by chain, side view.
PDB entry 1vfy coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of a phosphatidylinositol 3-phosphate-specific membrane-targeting motif, the FYVE domain of Vps27p.
Misra S, Hurley JH
Cell 97 657-66 (1999)
PMID: 10367894

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154097 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar protein sorting-associated protein 27 Chain: A
Molecule details ›
Chain: A
Length: 73 amino acids
Theoretical weight: 8.27 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40343 (Residues: 163-231; Coverage: 11%)
Gene names: DID7, GRD11, N2038, SSV17, VPL23, VPS27, VPT27, YNR006W
Sequence domains: FYVE zinc finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P1
Unit cell:
a: 24.09Å b: 26.57Å c: 31.61Å
α: 111.79° β: 92.7° γ: 105.7°
R-values:
R R work R free
0.174 0.174 0.181
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

51 review citations

Synthesis and function of 3-phosphorylated inositol lipids.
Vanhaesebroeck et al. (2001)
50 more

22 mentions without citation

The ESCRT complexes.
Hurley JH. (2010)
21 more