› 1vk1

X-ray diffraction
1.2Å resolution

Conserved hypothetical protein from Pyrococcus furiosus Pfu-392566-001

Released:
DOI: 10.2210/pdb1vk1/pdb
PDB entry 1vk1 coloured by chain, front view.
PDB entry 1vk1 coloured by chain, side view.
PDB entry 1vk1 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
(NZ)CH...O contacts assist crystallization of a ParB-like nuclease.
Shaw N, Cheng C, Tempel W, Chang J, Ng J, Wang XY, Perrett S, Rose J, Rao Z, Wang BC, Liu ZJ
BMC Struct Biol 7 46 (2007)
PMID: 17617922

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ParB/Sulfiredoxin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 27.99 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U3S5 (Residues: 1-242; Coverage: 100%)
Gene name: PF0380
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PO4 1 x PO4
Ligand NA 1 x NA
Ligand PEG 1 x PEG
1 modified residue:
Ligand MLY 9 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 115.148Å b: 40.099Å c: 65.671Å
α: 90° β: 122.29° γ: 90°
R-values:
R R work R free
0.166 0.165 0.175
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The "Sticky Patch" Model of Crystallization and Modification of Proteins for Enhanced Crystallizability.
Derewenda et al. (2017)
12 other articles

5 mentions without citation

Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS).
Hura et al. (2009)
4 more