› 1vk8

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF A PUTATIVE THIAMINE BIOSYNTHESIS/SALVAGE PROTEIN (TM0486) FROM THERMOTOGA MARITIMA AT 1.80 A RESOLUTION

Released:
DOI: 10.2210/pdb1vk8/pdb
PDB entry 1vk8 coloured by chain, front view.
PDB entry 1vk8 coloured by chain, side view.
PDB entry 1vk8 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
TM0486 from the hyperthermophilic anaerobe Thermotoga maritima is a thiamin-binding protein involved in response of the cell to oxidative conditions.
Dermoun Z, Foulon A, Miller MD, Harrington DJ, Deacon AM, Sebban-Kreuzer C, Roche P, Lafitte D, Bornet O, Wilson IA, Dolla A
J Mol Biol 400 463-76 (2010)
PMID: 20471400

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiamine-binding protein domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 106 amino acids
Theoretical weight: 12.42 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WYV6 (Residues: 1-94; Coverage: 100%)
Gene name: TM_0486
Sequence domains: Thiamine-binding protein
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand UNL 4 x UNL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P1
Unit cell:
a: 47.646Å b: 47.65Å c: 49.608Å
α: 73.8° β: 62.9° γ: 73.62°
R-values:
R R work R free
0.144 0.142 0.19
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Diversity of membrane transport proteins for vitamins in bacteria and archaea.
Jaehme et al. (2015)
1 more

3 mentions without citation

Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard.
Terwilliger et al. (2009)
2 more