› 1vkq

X-ray diffraction
1.6Å resolution

A re-determination of the structure of the triple mutant (K53,56,120M) of phospholipase A2 at 1.6A resolution using sulphur-SAS at 1.54A wavelength

Released:
DOI: 10.2210/pdb1vkq/pdb
PDB entry 1vkq coloured by chain, front view.
PDB entry 1vkq coloured by chain, side view.
PDB entry 1vkq coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
A redetermination of the structure of the triple mutant (K53,56,120M) of phospholipase A2 at 1.6 A resolution using sulfur-SAS at 1.54 A wavelength.
Sekar K, Rajakannan V, Velmurugan D, Yamane T, Thirumurugan R, Dauter M, Dauter Z
Acta Crystallogr D Biol Crystallogr 60 1586-90 (2004)
PMID: 15333929

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132750 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2 Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.82 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00593 (Residues: 23-145; Coverage: 95%)
Gene name: PLA2G1B
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand CL 1 x CL
Ligand MPD 3 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P3121
Unit cell:
a: 46.05Å b: 46.05Å c: 101.58Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.177 0.217
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

On the routine use of soft X-rays in macromolecular crystallography. Part V. Molecular replacement and anomalous scattering.
Unge et al. (2011)

2 mentions without citation

3dSS: 3D structural superposition.
Sumathi et al. (2006)
1 more