› 1vl8

X-ray diffraction
2.07Å resolution

Crystal structure of Gluconate 5-dehydrogenase (TM0441) from Thermotoga maritima at 2.07 A resolution

Released:
DOI: 10.2210/pdb1vl8/pdb
PDB entry 1vl8 coloured by chain, front view.
PDB entry 1vl8 coloured by chain, side view.
PDB entry 1vl8 coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
D-gluconate + NAD(P)(+) = 5-dehydro-D-gluconate + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-194537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxidoreductase, short chain dehydrogenase/reductase family Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 29.41 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WYS2 (Residues: 1-255; Coverage: 100%)
Gene name: TM_0441
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
2 bound ligands:
Ligand PO4 1 x PO4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P43212
Unit cell:
a: 123.504Å b: 123.504Å c: 122.323Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.15 0.18
Expression system: Escherichia coli

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