› 1vlc

X-ray diffraction
1.9Å resolution

Crystal structure of 3-isopropylmalate dehydrogenase (TM0556) from Thermotoga maritima at 1.90 A resolution

Released:
DOI: 10.2210/pdb1vlc/pdb
PDB entry 1vlc coloured by chain, front view.
PDB entry 1vlc coloured by chain, side view.
PDB entry 1vlc coloured by chain, top view.
Source organism: Thermotoga maritima
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
(1a) (2R,3S)-3-isopropylmalate + NAD(+) = (2S)-2-isopropyl-3-oxosuccinate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194565 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-isopropylmalate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 366 amino acids
Theoretical weight: 40.7 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WZ26 (Residues: 1-354; Coverage: 100%)
Gene names: TM_0556, leuB
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P321
Unit cell:
a: 118.78Å b: 118.78Å c: 56.699Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.188 0.218
Expression system: Escherichia coli

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