› 1vlr

X-ray diffraction
1.83Å resolution

Function and Biology Details

Reaction catalysed: 
A 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] + H(2)O = N(7)-methylguanosine 5'-phosphate + a 5'-diphospho-[mRNA]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190080 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
m7GpppX diphosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 40.5 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9DAR7 (Residues: 1-338; Coverage: 100%)
Gene names: Dcps, Dcs1, Hint5
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 6 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 65.399Å b: 59.231Å c: 100.14Å
α: 90° β: 103.8° γ: 90°
R-values:
R R work R free
0.161 0.158 0.201
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The complex enzymology of mRNA decapping: Enzymes of four classes cleave pyrophosphate bonds.
Kramer et al. (2019)
1 more

2 mentions without citation

Genome-wide CRISPR-Cas9 Screen Identifies Leukemia-Specific Dependence on a Pre-mRNA Metabolic Pathway Regulated by DCPS.
Yamauchi et al. (2018)
1 more