› 1vpo

X-ray diffraction
2.15Å resolution

Crystal Structure Analysis of the Anti-testosterone Fab in Complex with Testosterone

Released:
DOI: 10.2210/pdb1vpo/pdb
PDB entry 1vpo coloured by chain, front view.
PDB entry 1vpo coloured by chain, side view.
PDB entry 1vpo coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of an in vitro affinity- and specificity-matured anti-testosterone Fab in complex with testosterone. Improved affinity results from small structural changes within the variable domains.
Valjakka J, Hemminki A, Niemi S, Söderlund H, Takkinen K, Rouvinen J
J Biol Chem 277 44021-7 (2002)
PMID: 12196551

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-213105 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
anti-testosterone (light chain) Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24.15 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
anti-testosterone (heavy chain) Chain: H
Molecule details ›
Chain: H
Length: 221 amino acids
Theoretical weight: 23.58 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand TES 1 x TES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P62
Unit cell:
a: 82.252Å b: 82.252Å c: 120.059Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.211 0.274
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Synthetic antibodies: concepts, potential and practical considerations.
Miersch et al. (2012)
6 more

2 mentions without citation

A complete, multi-level conformational clustering of antibody complementarity-determining regions.
Nikoloudis et al. (2014)
1 more