› 1vpr

X-ray diffraction
1.8Å resolution

Crystal structure of a luciferase domain from the dinoflagellate Lingulodinium polyedrum

Released:
DOI: 10.2210/pdb1vpr/pdb
PDB entry 1vpr coloured by chain, front view.
PDB entry 1vpr coloured by chain, side view.
PDB entry 1vpr coloured by chain, top view.
Source organism: Lingulodinium polyedra
Primary publication:
Crystal structure of a pH-regulated luciferase catalyzing the bioluminescent oxidation of an open tetrapyrrole.
Schultz LW, Liu L, Cegielski M, Hastings JW
Proc. Natl. Acad. Sci. U.S.A. 102 1378-83 (2005)
PMID: 15665092

Function and Biology Details

Reaction catalysed: 
Dinoflagellate luciferin + O(2) = oxidized dinoflagellate luciferin + H(2)O + light
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131261 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dinoflagellate luciferase Chain: A
Molecule details ›
Chain: A
Length: 374 amino acids
Theoretical weight: 42.03 KDa
Source organism: Lingulodinium polyedra
Expression system: Escherichia coli
UniProt:
  • Canonical: O77206 (Residues: 866-1239; Coverage: 30%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 58.989Å b: 63.955Å c: 96.986Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.23
Expression system: Escherichia coli

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Citations

9 review citations

Bioluminescence in the sea.
Haddock et al. (2010)
8 more

4 mentions without citation

Bioluminescence and Photoreception in Unicellular Organisms: Light-Signalling in a Bio-Communication Perspective.
Timsit et al. (2021)
3 more