› 1vq2

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF T4-BACTERIOPHAGE DEOXYCYTIDYLATE DEAMINASE, MUTANT R115E

Released:
DOI: 10.2210/pdb1vq2/pdb
PDB entry 1vq2 coloured by chain, front view.
PDB entry 1vq2 coloured by chain, side view.
PDB entry 1vq2 coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Three-dimensional structure of the R115E mutant of T4-bacteriophage 2'-deoxycytidylate deaminase.
Almog R, Maley F, Maley GF, Maccoll R, Van Roey P
Biochemistry 43 13715-23 (2004)
PMID: 15504034

Function and Biology Details

Reaction catalysed: 
dCMP + H(2)O = dUMP + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-147577 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxycytidylate deaminase Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 21.2 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P16006 (Residues: 1-193; Coverage: 100%)
Gene name: CD
Sequence domains: Cytidine and deoxycytidylate deaminase zinc-binding region
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand DDN 1 x DDN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P6322
Unit cell:
a: 114.528Å b: 114.528Å c: 76.95Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.218 0.245
Expression system: Escherichia coli

Quick links

Citations

11 citation in other articles

Inference of macromolecular assemblies from crystalline state.
Krissinel et al. (2007)
10 more

5 mentions without citation

Structural biology of the purine biosynthetic pathway.
Zhang et al. (2008)
4 more