› 1vrv

Solution NMR

Structure of phosphorylated IIB (C384(SEP)) domain of the mannitol-specific permease enzyme II

Released:
DOI: 10.2210/pdb1vrv/pdb
PDB entry 1vrv coloured by chain, front view.
PDB entry 1vrv coloured by chain, side view.
PDB entry 1vrv coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Visualization of the phosphorylated active site loop of the cytoplasmic B domain of the mannitol transporter II(Mannitol) of the Escherichia coli phosphotransferase system by NMR spectroscopy and residual dipolar couplings.
Suh JY, Tang C, Cai M, Clore GM
J Mol Biol 353 1129-36 (2005)
PMID: 16219324

Function and Biology Details

Reaction catalysed: 
[Protein]-N(pi)-phospho-L-histidine + D-mannitol(Side 1) = [protein]-L-histidine + D-mannitol 1-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132711 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system mannitol-specific EIICBA component Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 11.03 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00550 (Residues: 375-475; Coverage: 16%)
Gene names: JW3573, b3599, mtlA
Sequence domains: PTS system, Lactose/Cellobiose specific IIB subunit
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

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Citations

1 review citation

Structure, dynamics and biophysics of the cytoplasmic protein-protein complexes of the bacterial phosphoenolpyruvate: sugar phosphotransferase system.
Clore et al. (2013)
7 other articles