› 1vs3

X-ray diffraction
2.25Å resolution

Crystal Structure of the tRNA Pseudouridine Synthase TruA From Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb1vs3/pdb
PDB entry 1vs3 coloured by chain, front view.
PDB entry 1vs3 coloured by chain, side view.
PDB entry 1vs3 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Crystal structure of tRNA pseudouridine synthase TruA from Thermus thermophilus HB8.
Dong X, Bessho Y, Shibata R, Nishimoto M, Shirouzu M, Kuramitsu S, Yokoyama S
RNA Biol 3 115-22 (2006)
PMID: 17114947

Function and Biology Details

Reaction catalysed: 
tRNA uridine(38-40) = tRNA pseudouridine(38-40)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177902 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA pseudouridine synthase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 27.75 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SHU9 (Residues: 1-249; Coverage: 98%)
Gene names: TTHA1631, truA
Sequence domains: tRNA pseudouridine synthase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A, SPRING-8 BEAMLINE BL26B1
Spacegroup: P41212
Unit cell:
a: 91.53Å b: 91.53Å c: 163.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.234
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structure, dynamics, and function of RNA modification enzymes.
Ishitani et al. (2008)
2 more

3 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
2 more