› 1vsr

X-ray diffraction
1.8Å resolution

VERY SHORT PATCH REPAIR (VSR) ENDONUCLEASE FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1vsr/pdb
PDB entry 1vsr coloured by chain, front view.
PDB entry 1vsr coloured by chain, side view.
PDB entry 1vsr coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystallographic and functional studies of very short patch repair endonuclease.
Tsutakawa SE, Muto T, Kawate T, Jingami H, Kunishima N, Ariyoshi M, Kohda D, Nakagawa M, Morikawa K
Mol Cell 3 621-8 (1999)
PMID: 10360178

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140586 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA mismatch endonuclease Vsr Chain: A
Molecule details ›
Chain: A
Length: 136 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09184 (Residues: 21-156; Coverage: 87%)
Gene names: JW1943, b1960, vsr
Sequence domains: DNA mismatch endonuclease Vsr
Structure domains: VSR Endonuclease

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: P212121
Unit cell:
a: 35.02Å b: 54.24Å c: 73.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

DNA base repair--recognition and initiation of catalysis.
Dalhus et al. (2009)
6 more

9 mentions without citation

FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain.
Boehm et al. (2017)
8 more