› 1vwa

X-ray diffraction
1.85Å resolution

STREPTAVIDIN-FSHPQNT

Released:
DOI: 10.2210/pdb1vwa/pdb
PDB entry 1vwa coloured by chain, front view.
PDB entry 1vwa coloured by chain, side view.
PDB entry 1vwa coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
In crystals of complexes of streptavidin with peptide ligands containing the HPQ sequence the pKa of the peptide histidine is less than 3.0.
Katz BA, Cass RT
J Biol Chem 272 13220-8 (1997)
PMID: 9148939

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149709 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Streptavidin Chains: B, D
Molecule details ›
Chains: B, D
Length: 123 amino acids
Theoretical weight: 12.97 KDa
Source organism: Streptomyces avidinii
UniProt:
  • Canonical: P22629 (Residues: 37-159; Coverage: 77%)
Sequence domains: Avidin family
Structure domains: Avidin-like
PEPTIDE LIGAND CONTAINING HPQ Chains: M, P
Molecule details ›
Chains: M, P
Length: 7 amino acids
Theoretical weight: 831 Da
Source organism: Streptomyces avidinii
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I222
Unit cell:
a: 97.03Å b: 107.47Å c: 48.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.259
Expression system: Not provided

Quick links

Citations

1 review citation

Streptavidin-binding and -dimerizing ligands discovered by phage display, topochemistry, and structure-based design.
Katz BA. (1999)
13 other articles

3 mentions without citation

Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site.
Korndörfer et al. (2002)
2 more