› 1vyj

X-ray diffraction
2.8Å resolution

Structural and biochemical studies of human PCNA complexes provide the basis for association with CDK/cyclin and rationale for inhibitor design

Released:
DOI: 10.2210/pdb1vyj/pdb
PDB entry 1vyj coloured by chain, front view.
PDB entry 1vyj coloured by chain, side view.
PDB entry 1vyj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and biochemical studies of human proliferating cell nuclear antigen complexes provide a rationale for cyclin association and inhibitor design.
Kontopidis G, Wu SY, Zheleva DI, Taylor P, McInnes C, Lane DP, Fischer PM, Walkinshaw MD
Proc Natl Acad Sci U S A 102 1871-6 (2005)
PMID: 15681588

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146049 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 261 amino acids
Theoretical weight: 28.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
SMALL PEPTIDE SAVLQKKITDYFHPKK Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 16 amino acids
Theoretical weight: 1.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P3121
Unit cell:
a: 119.101Å b: 119.101Å c: 305.817Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.176 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
Putnam et al. (2007)
9 more

12 mentions without citation

Proliferating cell nuclear antigen acts as a cytoplasmic platform controlling human neutrophil survival.
Witko-Sarsat et al. (2010)
11 more