› 1vzt

X-ray diffraction
2Å resolution

ROLES OF INDIVIDUAL RESIDUES OF ALPHA-1,3 GALACTOSYLTRANSFERASES IN SUBSTRATE BINDING AND CATALYSIS

Released:
DOI: 10.2210/pdb1vzt/pdb
PDB entry 1vzt coloured by chain, front view.
PDB entry 1vzt coloured by chain, side view.
PDB entry 1vzt coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Roles of individual enzyme-substrate interactions by alpha-1,3-galactosyltransferase in catalysis and specificity.
Zhang Y, Swaminathan GJ, Deshpande A, Boix E, Natesh R, Xie Z, Acharya KR, Brew K
Biochemistry 42 13512-21 (2003)
PMID: 14621997

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyllactosaminide alpha-1,3-galactosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 33.96 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14769 (Residues: 80-368; Coverage: 79%)
Gene name: GGTA1
Sequence domains: Glycosyltransferase family 6
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand UDP 2 x UDP
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P21
Unit cell:
a: 45.058Å b: 94.211Å c: 94.57Å
α: 90° β: 98.96° γ: 90°
R-values:
R R work R free
0.193 0.193 0.22
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
6 more