› 1w15

X-ray diffraction
1.93Å resolution

rat synaptotagmin 4 C2B domain in the presence of calcium

Released:
DOI: 10.2210/pdb1w15/pdb
PDB entry 1w15 coloured by chain, front view.
PDB entry 1w15 coloured by chain, side view.
PDB entry 1w15 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural basis for the evolutionary inactivation of Ca2+ binding to synaptotagmin 4.
Dai H, Shin OH, Machius M, Tomchick DR, Südhof TC, Rizo J
Nat Struct Mol Biol 11 844-9 (2004)
PMID: 15311271

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156151 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Synaptotagmin-4 Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 16.62 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50232 (Residues: 286-425; Coverage: 33%)
Gene name: Syt4
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

3 bound ligands:
Ligand CA 3 x CA
Ligand NA 2 x NA
Ligand CL 9 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P6322
Unit cell:
a: 91.49Å b: 91.49Å c: 122.142Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.204 0.233
Expression system: Escherichia coli BL21(DE3)

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Citations

17 review citations

Astrocytes, from brain glue to communication elements: the revolution continues.
Volterra et al. (2005)
16 more

1 mention without citation

Capture of Dense Core Vesicles at Synapses by JNK-Dependent Phosphorylation of Synaptotagmin-4.
Bharat et al. (2017)