› 1w18

X-ray diffraction
2.5Å resolution

Crystal Structure of levansucrase from Gluconacetobacter diazotrophicus

Released:
DOI: 10.2210/pdb1w18/pdb
PDB entry 1w18 coloured by chain, front view.
PDB entry 1w18 coloured by chain, side view.
PDB entry 1w18 coloured by chain, top view.
Source organism: Gluconacetobacter diazotrophicus
Primary publication:
Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus.
Martínez-Fleites C, Ortíz-Lombardía M, Pons T, Tarbouriech N, Taylor EJ, Arrieta JG, Hernández L, Davies GJ
Biochem J 390 19-27 (2005)
PMID: 15869470

Function and Biology Details

Reaction catalysed: 
Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175005 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Levansucrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 493 amino acids
Theoretical weight: 53.98 KDa
Source organism: Gluconacetobacter diazotrophicus
UniProt:
  • Canonical: Q43998 (Residues: 62-554; Coverage: 89%)
Gene name: lsdA
Sequence domains: Levansucrase/Invertase
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 53.803Å b: 119.391Å c: 215.101Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.25

Quick links

Citations

11 review citations

Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria.
van Hijum et al. (2006)
10 more

9 mentions without citation

Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus.
Martínez-Fleites et al. (2005)
8 more