› 1w2t

X-ray diffraction
1.87Å resolution

beta-fructosidase from Thermotoga maritima in complex with raffinose

Released:
DOI: 10.2210/pdb1w2t/pdb
PDB entry 1w2t coloured by chain, front view.
PDB entry 1w2t coloured by chain, side view.
PDB entry 1w2t coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Crystal structure of inactivated Thermotoga maritima invertase in complex with the trisaccharide substrate raffinose.
Alberto F, Jordi E, Henrissat B, Czjzek M
Biochem J 395 457-62 (2006)
PMID: 16411890

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128504 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-fructosidase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 432 amino acids
Theoretical weight: 49.87 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O33833 (Residues: 1-432; Coverage: 100%)
Gene names: TM_1414, bfrA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU, GLA
Carbohydrate polymer
2 bound ligands:
Ligand CIT 1 x CIT
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 94.5Å b: 114.7Å c: 130Å
α: 90° β: 98.9° γ: 90°
R-values:
R R work R free
0.199 0.198 0.229
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Structural insights into glycoside hydrolase family 32 and 68 enzymes: functional implications.
Lammens et al. (2009)
1 more

10 mentions without citation

The carbohydrate-active enzymes database (CAZy) in 2013.
Lombard et al. (2014)
9 more