› 1w2y

X-ray diffraction
1.65Å resolution

The crystal structure of a complex of Campylobacter jejuni dUTPase with substrate analogue dUpNHp

Released:
DOI: 10.2210/pdb1w2y/pdb
PDB entry 1w2y coloured by chain, front view.
PDB entry 1w2y coloured by chain, side view.
PDB entry 1w2y coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
The crystal structure of a complex of Campylobacter jejuni dUTPase with substrate analogue sheds light on the mechanism and suggests the "basic module" for dimeric d(C/U)TPases.
Moroz OV, Harkiolaki M, Galperin MY, Vagin AA, González-Pacanowska D, Wilson KS
J Mol Biol 342 1583-97 (2004)
PMID: 15364583

Function and Biology Details

Reaction catalysed: 
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171012 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dUTPase Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 27.05 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0P8G4 (Residues: 1-229; Coverage: 100%)
Gene names: Cj1451, dut
Sequence domains: dUTPase
Structure domains: Type II deoxyuridine triphosphatase

Ligands and Environments

2 bound ligands:
Ligand DUN 2 x DUN
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 66.963Å b: 70.629Å c: 92.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.194
Expression system: Escherichia coli

Quick links

Citations

6 review citations

DNA base damage recognition and removal: new twists and grooves.
Huffman et al. (2005)
5 more

8 mentions without citation

MrBUMP: an automated pipeline for molecular replacement.
Keegan et al. (2008)
7 more