› 1w4j

Solution NMR

Peripheral-subunit binding domains from mesophilic, thermophilic, and hyperthermophilic bacteria fold by ultrafast, apparently two-state transitions

Released:
DOI: 10.2210/pdb1w4j/pdb
PDB entry 1w4j coloured by chain, ensemble of 20 models, front view.
PDB entry 1w4j coloured by chain, ensemble of 20 models, side view.
PDB entry 1w4j coloured by chain, ensemble of 20 models, top view.
Source organism: Pyrobaculum aerophilum
Primary publication:
Ultra-fast barrier-limited folding in the peripheral subunit-binding domain family.
Ferguson N, Sharpe TD, Schartau PJ, Sato S, Allen MD, Johnson CM, Rutherford TJ, Fersht AR
J Mol Biol 353 427-46 (2005)
PMID: 16168437

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate dehydrogenase E2 (Dihydrolipoamide acetyltransferase) Chain: A
Molecule details ›
Chain: A
Length: 51 amino acids
Theoretical weight: 5.26 KDa
Source organism: Pyrobaculum aerophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZUR6 (Residues: 93-141; Coverage: 13%)
Gene name: PAE2648
Sequence domains: e3 binding domain
Structure domains: E3-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

The protein folding problem.
Dill et al. (2008)
12 more

2 mentions without citation

Cooperative folding kinetics of BBL protein and peripheral subunit-binding domain homologues.
Yu et al. (2008)
1 more