› 1w4n

X-ray diffraction
1.65Å resolution

AGAO covalent complex with Tranylcypromine

Released:
DOI: 10.2210/pdb1w4n/pdb
PDB entry 1w4n coloured by chain, front view.
PDB entry 1w4n coloured by chain, side view.
PDB entry 1w4n coloured by chain, top view.
Source organism: Arthrobacter globiformis
Primary publication:
Complexes of the copper-containing amine oxidase from Arthrobacter globiformis with the inhibitors benzylhydrazine and tranylcypromine.
Langley DB, Trambaiolo DM, Duff AP, Dooley DM, Freeman HC, Guss JM
Acta Crystallogr Sect F Struct Biol Cryst Commun 64 577-83 (2008)
PMID: 18607080

Function and Biology Details

Reaction catalysed: 
RCH(2)NH(2) + H(2)O + O(2) = RCHO + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylethylamine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 646 amino acids
Theoretical weight: 71.88 KDa
Source organism: Arthrobacter globiformis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46881 (Residues: 3-638; Coverage: 100%)
Sequence domains: Copper amine oxidase, enzyme domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CU 2 x CU
Ligand NA 2 x NA
Ligand SO4 2 x SO4
Ligand GOL 9 x GOL
1 modified residue:
Ligand TCQ 2 x TCQ

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Unit cell:
a: 158.113Å b: 63.001Å c: 184.49Å
α: 90° β: 112.04° γ: 90°
R-values:
R R work R free
0.172 0.171 0.199
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Comparing hydrazine-derived reactive groups as inhibitors of quinone-dependent amine oxidases.
Burke et al. (2017)
2 more

2 mentions without citation

Copper active sites in biology.
Solomon et al. (2014)
1 more