› 1w4z

X-ray diffraction
2.5Å resolution

Structure of actinorhodin polyketide (actIII) Reductase

Released:
DOI: 10.2210/pdb1w4z/pdb
PDB entry 1w4z coloured by chain, front view.
PDB entry 1w4z coloured by chain, side view.
PDB entry 1w4z coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
The crystal structure of the actIII actinorhodin polyketide reductase: proposed mechanism for ACP and polyketide binding.
Hadfield AT, Limpkin C, Teartasin W, Simpson TJ, Crosby J, Crump MP
Structure 12 1865-75 (2004)
PMID: 15458634

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative ketoacyl reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 281 amino acids
Theoretical weight: 29.46 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16544 (Residues: 1-261; Coverage: 100%)
Gene names: SCBAC28G1.12c, SCO5086, actIII
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand NAP 2 x NAP
Ligand FMT 9 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P3221
Unit cell:
a: 103.43Å b: 103.43Å c: 122.823Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Type II polyketide synthases: gaining a deeper insight into enzymatic teamwork.
Hertweck et al. (2007)
10 more

2 mentions without citation

Insights into the Role of Ketoreductases in the Biosynthesis of Partially Reduced Bacterial Aromatic Polyketides.
Husain et al. (2020)
1 more