› 1w5i

X-ray diffraction
2.3Å resolution

ABA does not affect topology of pLI.

Released:
DOI: 10.2210/pdb1w5i/pdb
PDB entry 1w5i coloured by chain, front view.
PDB entry 1w5i coloured by chain, side view.
PDB entry 1w5i coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure-based engineering of internal cavities in coiled-coil peptides.
Yadav MK, Redman JE, Leman LJ, Alvarez-Gutiérrez JM, Zhang Y, Stout CD, Ghadiri MR
Biochemistry 44 9723-32 (2005)
PMID: 16008357

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136532 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 33 amino acids
Theoretical weight: 4.05 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P03069 (Residues: 249-281; Coverage: 12%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C
Sequence domains: Basic region leucine zipper

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P4132
Unit cell:
a: 79.222Å b: 79.222Å c: 79.222Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.231 0.295
Expression system: Not provided

Quick links

Citations

2 review citations

Molecular recognition with designed peptides and proteins.
Cooper et al. (2005)
1 more

3 mentions without citation

Coiled coils at the edge of configurational heterogeneity. Structural analyses of parallel and antiparallel homotetrameric coiled coils reveal configurational sensitivity to a single solvent-exposed amino acid substitution.
Yadav et al. (2006)
2 more