› 1w5s

X-ray diffraction
2.4Å resolution

Structure of the Aeropyrum Pernix ORC2 protein (ADP form)

Released:
DOI: 10.2210/pdb1w5s/pdb
PDB entry 1w5s coloured by chain, front view.
PDB entry 1w5s coloured by chain, side view.
PDB entry 1w5s coloured by chain, top view.
Source organism: Aeropyrum pernix
Primary publication:
Conformational changes induced by nucleotide binding in Cdc6/ORC from Aeropyrum pernix.
Singleton MR, Morales R, Grainge I, Cook N, Isupov MN, Wigley DB
J Mol Biol 343 547-57 (2004)
PMID: 15465044

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195496 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ORC1-type DNA replication protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 412 amino acids
Theoretical weight: 46.3 KDa
Source organism: Aeropyrum pernix
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9YFU8 (Residues: 2-410; Coverage: 100%)
Gene names: APE_0152.1, orc2
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: C2221
Unit cell:
a: 67.495Å b: 101.911Å c: 255.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.212 0.286
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Evolutionary relationships and structural mechanisms of AAA+ proteins.
Erzberger et al. (2006)
15 more

4 mentions without citation

Crystal structure of the eukaryotic origin recognition complex.
Bleichert et al. (2015)
3 more