› 1w69

X-ray diffraction
2.2Å resolution

Crystal Structure of Mouse Ribonucleotide Reductase Subunit R2 under Reducing Conditions. A Fully Occupied Dinuclear Iron Cluster and Bound Acetate.

Released:
DOI: 10.2210/pdb1w69/pdb
PDB entry 1w69 coloured by chain, front view.
PDB entry 1w69 coloured by chain, side view.
PDB entry 1w69 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structural studies of changes in the native dinuclear iron center of ribonucleotide reductase protein R2 from mouse.
Strand KR, Karlsen S, Kolberg M, Røhr AK, Görbitz CH, Andersson KK
J Biol Chem 279 46794-801 (2004)
PMID: 15322079

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145687 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase subunit M2 Chain: A
Molecule details ›
Chain: A
Length: 390 amino acids
Theoretical weight: 45.15 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11157 (Residues: 1-390; Coverage: 100%)
Gene name: Rrm2
Sequence domains: Ribonucleotide reductase, small chain
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

2 bound ligands:
Ligand FE2 2 x FE2
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: C2221
Unit cell:
a: 75.993Å b: 107.454Å c: 91.929Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Targeting ribonucleotide reductase for cancer therapy.
Shao et al. (2013)
3 more

4 mentions without citation

Structural analysis of specific metal chelating inhibitor binding to the endonuclease domain of influenza pH1N1 (2009) polymerase.
Kowalinski et al. (2012)
3 more