› 1w6c

X-ray diffraction
2.2Å resolution

AGAO holoenzyme in a small cell, at 2.2 angstroms

Released:
DOI: 10.2210/pdb1w6c/pdb
PDB entry 1w6c coloured by chain, front view.
PDB entry 1w6c coloured by chain, side view.
PDB entry 1w6c coloured by chain, top view.
Source organism: Arthrobacter globiformis
Primary publication:
The copper-containing amine oxidase from Arthrobacter globiformis: refinement at 1.55 and 2.20 A resolution in two crystal forms.
Langley DB, Duff AP, Freeman HC, Guss JM
Acta Crystallogr Sect F Struct Biol Cryst Commun 62 1052-7 (2006)
PMID: 17077478

Function and Biology Details

Reaction catalysed: 
RCH(2)NH(2) + H(2)O + O(2) = RCHO + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylethylamine oxidase Chain: A
Molecule details ›
Chain: A
Length: 646 amino acids
Theoretical weight: 71.76 KDa
Source organism: Arthrobacter globiformis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46881 (Residues: 3-638; Coverage: 100%)
Sequence domains: Copper amine oxidase, enzyme domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CU 2 x CU
Ligand NA 1 x NA
1 modified residue:
Ligand TPQ 1 x TPQ

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 158.041Å b: 64.061Å c: 69.693Å
α: 90° β: 111.74° γ: 90°
R-values:
R R work R free
0.175 0.172 0.219
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

Genetics of the glutamate-mediated methylamine utilization pathway in the facultative methylotrophic beta-proteobacterium Methyloversatilis universalis FAM5.
Latypova et al. (2010)
2 more

3 mentions without citation

Copper active sites in biology.
Solomon et al. (2014)
2 more