› 1w6t

X-ray diffraction
2.1Å resolution

Crystal Structure Of Octameric Enolase From Streptococcus pneumoniae

Released:
DOI: 10.2210/pdb1w6t/pdb
PDB entry 1w6t coloured by chain, front view.
PDB entry 1w6t coloured by chain, side view.
PDB entry 1w6t coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Plasmin(ogen)-binding alpha-enolase from Streptococcus pneumoniae: crystal structure and evaluation of plasmin(ogen)-binding sites.
Ehinger S, Schubert WD, Bergmann S, Hammerschmidt S, Heinz DW
J Mol Biol 343 997-1005 (2004)
PMID: 15476816

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188955 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 444 amino acids
Theoretical weight: 48.41 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q97QS2 (Residues: 1-434; Coverage: 100%)
Gene names: SP_1128, eno
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand 2PE 2 x 2PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: I4
Unit cell:
a: 143.702Å b: 143.702Å c: 100.579Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.148 0.187
Expression system: Escherichia coli BL21

Quick links

Citations

18 review citations

α-Enolase: a promising therapeutic and diagnostic tumor target.
Capello et al. (2011)
17 more

22 mentions without citation

Moonlighting Proteins in the Fuzzy Logic of Cellular Metabolism.
Liu et al. (2020)
21 more