› 1w78

X-ray diffraction
1.82Å resolution

E.coli FolC in complex with DHPP and ADP

Released:
DOI: 10.2210/pdb1w78/pdb
PDB entry 1w78 coloured by chain, front view.
PDB entry 1w78 coloured by chain, side view.
PDB entry 1w78 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Escherichia coli FolC structure reveals an unexpected dihydrofolate binding site providing an attractive target for anti-microbial therapy.
Mathieu M, Debousker G, Vincent S, Viviani F, Bamas-Jacques N, Mikol V
J Biol Chem 280 18916-22 (2005)
PMID: 15705579

Function and Biology Details

Reactions catalysed: 
ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate
ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140011 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate synthase/folylpolyglutamate synthase Chain: A
Molecule details ›
Chain: A
Length: 422 amino acids
Theoretical weight: 45.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08192 (Residues: 1-422; Coverage: 100%)
Gene names: JW2312, b2315, dedC, folC
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand PD8 1 x PD8
Ligand MG 2 x MG
Ligand SO4 1 x SO4
Ligand ADP 1 x ADP
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P212121
Unit cell:
a: 64.368Å b: 80.62Å c: 92.829Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.165 0.203
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structure, function and dynamics in the mur family of bacterial cell wall ligases.
Smith CA. (2006)
4 more

9 mentions without citation

Utility of the Biosynthetic Folate Pathway for Targets in Antimicrobial Discovery.
Bourne CR. (2014)
8 more