› 1w90

X-ray diffraction
2.5Å resolution

CBM29-2 mutant D114A: Probing the Mechanism of Ligand Recognition by Family 29 Carbohydrate Binding Modules

Released:
DOI: 10.2210/pdb1w90/pdb
PDB entry 1w90 coloured by chain, front view.
PDB entry 1w90 coloured by chain, side view.
PDB entry 1w90 coloured by chain, top view.
Source organism: Piromyces sp. 'equi'
Primary publication:
Probing the mechanism of ligand recognition in family 29 carbohydrate-binding modules.
Flint J, Bolam DN, Nurizzo D, Taylor EJ, Williamson MP, Walters C, Davies GJ, Gilbert HJ
J Biol Chem 280 23718-26 (2005)
PMID: 15784618

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189898 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CBM10 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 17.17 KDa
Source organism: Piromyces sp. 'equi'
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9C171 (Residues: 334-478; Coverage: 32%)
Gene name: ncp1
Structure domains: Galactose-binding lectin

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P43212
Unit cell:
a: 93.19Å b: 93.19Å c: 82.354Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Carbohydrate-binding domains: multiplicity of biological roles.
Guillén et al. (2010)
19 other articles