› 1wbx

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURES OF MURINE MHC CLASS I H-2 Db AND Kb MOLECULES IN COMPLEX WITH CTL EPITOPES FROM INFLUENZA A VIRUS: IMPLICATIONS FOR TCR REPERTOIRE SELECTION AND IMMUNODOMINANCE

Released:
DOI: 10.2210/pdb1wbx/pdb
PDB entry 1wbx coloured by chain, front view.
PDB entry 1wbx coloured by chain, side view.
PDB entry 1wbx coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of murine MHC Class I H-2 D(b) and K(b) molecules in complex with CTL epitopes from influenza A virus: implications for TCR repertoire selection and immunodominance.
Meijers R, Lai CC, Yang Y, Liu JH, Zhong W, Wang JH, Reinherz EL
J Mol Biol 345 1099-110 (2005)
PMID: 15644207

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-134990 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, D-B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 32.09 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01899 (Residues: 25-300; Coverage: 82%)
Gene name: H2-D1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Hemagglutinin HA2 chain Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.15 KDa
Source organism: Influenza A virus
Expression system: Not provided
UniProt:
  • Canonical: P26140 (Residues: 468-477; Coverage: 2%)
Gene name: HA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 56.759Å b: 56.759Å c: 275.996Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.198 0.255
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

7 review citations

Confronting complexity: real-world immunodominance in antiviral CD8+ T cell responses.
Yewdell JW. (2006)
6 more

5 mentions without citation

DockTope: a Web-based tool for automated pMHC-I modelling.
Rigo et al. (2015)
4 more