› 1wbz

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURES OF MURINE MHC CLASS I H-2 Db AND Kb MOLECULES IN COMPLEX WITH CTL EPITOPES FROM INFLUENZA A VIRUS: IMPLICATIONS FOR TCR REPERTOIRE SELECTION AND IMMUNODOMINANCE

Released:
DOI: 10.2210/pdb1wbz/pdb
PDB entry 1wbz coloured by chain, front view.
PDB entry 1wbz coloured by chain, side view.
PDB entry 1wbz coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of murine MHC Class I H-2 D(b) and K(b) molecules in complex with CTL epitopes from influenza A virus: implications for TCR repertoire selection and immunodominance.
Meijers R, Lai CC, Yang Y, Liu JH, Zhong W, Wang JH, Reinherz EL
J Mol Biol 345 1099-110 (2005)
PMID: 15644207

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135049 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 275 amino acids
Theoretical weight: 31.78 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01901 (Residues: 22-296; Coverage: 79%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, D
Molecule details ›
Chains: B, D
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
RNA-directed RNA polymerase catalytic subunit Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 9 amino acids
Theoretical weight: 1.04 KDa
Source organism: Influenza A virus
Expression system: Not provided
UniProt:
  • Canonical: P26144 (Residues: 107-115; Coverage: 6%)
Gene name: PB1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 89.166Å b: 91.256Å c: 67.183Å
α: 90° β: 111.37° γ: 90°
R-values:
R R work R free
0.21 0.208 0.254
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

7 review citations

Confronting complexity: real-world immunodominance in antiviral CD8+ T cell responses.
Yewdell JW. (2006)
6 more

4 mentions without citation

pDOCK: a new technique for rapid and accurate docking of peptide ligands to Major Histocompatibility Complexes.
Khan et al. (2010)
3 more