› 1wcf

X-ray diffraction
1.54Å resolution

1.54 A CRYSTAL STRUCTURE OF RV3628, MYCOBACTERIUM TUBERCULOSIS INORGANIC PYROPHOSPHATASE (PPASE) AT PH7.0

Released:
DOI: 10.2210/pdb1wcf/pdb
PDB entry 1wcf coloured by chain, front view.
PDB entry 1wcf coloured by chain, side view.
PDB entry 1wcf coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structure of the Mycobacterium tuberculosis soluble inorganic pyrophosphatase Rv3628 at pH 7.0.
Benini S, Wilson K
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 866-70 (2011)
PMID: 21821883

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161596 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chain: A
Molecule details ›
Chain: A
Length: 171 amino acids
Theoretical weight: 19.49 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WI55 (Residues: 1-162; Coverage: 100%)
Gene names: MTCY15C10.24, Rv3628, ppa
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

2 bound ligands:
Ligand K 2 x K
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6322
Unit cell:
a: 96.902Å b: 96.902Å c: 103.312Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.156 0.17
Expression system: Escherichia coli BL21(DE3)

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Citations

4 citation in other articles

Mycobacterium tuberculosis Rv3628 drives Th1-type T cell immunity via TLR2-mediated activation of dendritic cells and displays vaccine potential against the hyper-virulent Beijing K strain.
Kim et al. (2016)
3 more

1 mention without citation

Structure of the Mycobacterium tuberculosis soluble inorganic pyrophosphatase Rv3628 at pH 7.0.
Benini et al. (2011)