› 1wcw

X-ray diffraction
1.3Å resolution

Crystal Structure of Uroporphyrinogen III Synthase from an Extremely Thermophilic Bacterium Thermus thermophilus HB8 (Wild type, Native, Form-1 crystal)

Released:
DOI: 10.2210/pdb1wcw/pdb
PDB entry 1wcw coloured by chain, front view.
PDB entry 1wcw coloured by chain, side view.
PDB entry 1wcw coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Mizohata E, Matsuura T, Sakai H, Murayama K, Terada T, Shirouzu M, Kuramitsu S, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
Hydroxymethylbilane = uroporphyrinogen III + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178087 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetrapyrrole biosynthesis uroporphyrinogen III synthase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 28.44 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SKH2 (Residues: 2-253; Coverage: 100%)
Gene name: TTHA0671
Sequence domains: Uroporphyrinogen-III synthase HemD
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P21
Unit cell:
a: 35.546Å b: 66.309Å c: 55.677Å
α: 90° β: 91.784° γ: 90°
R-values:
R R work R free
0.188 0.188 0.216
Expression system: Escherichia coli BL21(DE3)

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