› 1wcx

X-ray diffraction
2Å resolution

Crystal Structure of Mutant Uroporphyrinogen III Synthase from an Extremely Thermophilic Bacterium Thermus thermophilus HB8 (L75M/I193M/L248M, SeMet derivative, Form-1 crystal)

Released:
DOI: 10.2210/pdb1wcx/pdb
PDB entry 1wcx coloured by chain, front view.
PDB entry 1wcx coloured by chain, side view.
PDB entry 1wcx coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Mizohata E, Matsuura T, Murayama K, Sakai H, Terada T, Shirouzu M, Kuramitsu S, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
Hydroxymethylbilane = uroporphyrinogen III + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178087 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetrapyrrole biosynthesis uroporphyrinogen III synthase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 28.73 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SKH2 (Residues: 2-253; Coverage: 100%)
Gene name: TTHA0671
Sequence domains: Uroporphyrinogen-III synthase HemD
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P21
Unit cell:
a: 35.548Å b: 66.105Å c: 55.857Å
α: 90° β: 91.4° γ: 90°
R-values:
R R work R free
0.19 0.19 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links