› 1wcy

X-ray diffraction
2.2Å resolution

Crystal Structure Of Human Dipeptidyl Peptidase IV (DPPIV) Complex With Diprotin A

Released:
DOI: 10.2210/pdb1wcy/pdb
PDB entry 1wcy coloured by chain, front view.
PDB entry 1wcy coloured by chain, side view.
PDB entry 1wcy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of human dipeptidyl peptidase IV (DPPIV) complex with diprotin A.
Hiramatsu H, Yamamoto A, Kyono K, Higashiyama Y, Fukushima C, Shima H, Sugiyama S, Inaka K, Shimizu R
Biol Chem 385 561-4 (2004)
PMID: 15255191

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151018 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Dipeptidyl peptidase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 740 amino acids
Theoretical weight: 85.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P27487 (Residues: 33-766; Coverage: 96%)
Gene names: ADCP2, CD26, DPP4
Sequence domains:
Structure domains:
Diprotin A Chains: C, D
Molecule details ›
Chains: C, D
Length: 3 amino acids
Theoretical weight: 341 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 5 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32B2
Spacegroup: P212121
Unit cell:
a: 118.02Å b: 126.06Å c: 136.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.265
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

22 citation in other articles

Skin fibrosis. Identification and isolation of a dermal lineage with intrinsic fibrogenic potential.
Rinkevich et al. (2015)
21 more

12 mentions without citation

Discovery of Food-Derived Dipeptidyl Peptidase IV Inhibitory Peptides: A Review.
Liu et al. (2019)
11 more